PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Colon: An at least moderate cytoplasmic CEA staining should be seen in the vast majority of columnar epithelial cells.
Negative Control
Colon: CEA staining should not be seen in stromal cells or smooth muscle.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human CEA protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
carcinoembryonic antigen related cell adhesion molecule 5 , CD66e , CEA
Cellular Localization
Cell membrane , Apical cell membrane , Cell surface
Background
This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Database
Research Area
DATA IMAGES
![Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399](/templates/default/js/lightbox/images/loading.gif "Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399")
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GTX04399 IHC-P Image
IHC-P analysis of human colon tissue using GTX04399 CD66e antibody [MSVA-465R] HistoMAX™.
Moderate to strong CD66e immunostaining in the epithelial cells of the colon. Inflammatory and smooth muscle cells remain negative.
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GTX04399 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04399 CD66e antibody [MSVA-465R] HistoMAX™.
Strong CD66e immunostaining of the superfical layers of the squamous epithelium of the tonsil surface. The basal epithelial cell layers and inflammatory cells are CD66e negative.
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GTX04399 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma (COAD) tissue using GTX04399 CD66e antibody [MSVA-465R] HistoMAX™.
Colorectal adenocarcinoma showing strong CD66e immunostaining in tumor cells.
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REFERENCE
There are currently no references for CD66e antibody [MSVA-465R] HistoMAX™ (GTX04399). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD66e antibody [MSVA-465R] HistoMAX™ (GTX04399). Be the first to share your experience with this product.
![Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399](/upload/website/prouct_img/small/GTX04399/GTX04399_20230728_IHC-P_25_23072722_786.webp "Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399")
![Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399](/upload/website/prouct_img/small/GTX04399/GTX04399_20230728_IHC-P_155_23072722_125.webp "Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399")
![Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399](/upload/website/prouct_img/small/GTX04399/GTX04399_20230728_IHC-P_285_23072723_847.webp "Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399")
![Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399](/upload/website/prouct_img/normal/GTX04399/GTX04399_20230728_IHC-P_25_23072722_786.webp "Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399")
![Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399](/upload/website/prouct_img/normal/GTX04399/GTX04399_20230728_IHC-P_155_23072722_125.webp "Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399")
![Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399](/upload/website/prouct_img/normal/GTX04399/GTX04399_20230728_IHC-P_285_23072723_847.webp "Anti-CD66e antibody [MSVA-465R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04399")