CD68 antibody [ED1]

Cat. No. GTX41868

Reference 13

Host	Mouse
Clonality	Monoclonal
Clone Name	ED1
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, RIA
Reactivity	Human, Rat, Bovine
Package	100 μg ($449)

See all CD68 products

View product citations for antibody GTX41868 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	1/50-1/100
IP	Assay dependent
RIA	Assay dependent
Note : IHC-P Recommend a proteolytic antigen retrieval. FACS Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl.

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat spleen cells

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11163177

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD68 molecule , GP110 , LAMP4 , SCARD1

Background

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Gene ID: 968 CD68
UniProt: P34810 CD68

Research Area

DATA IMAGES

GTX41868 IHC-P Image

IHC analysis of rat liver, with induced hepatocellular damage tissue using GTX41868 CD68 antibody [ED1].

GTX41868 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody (red n A) and Mouse anti Rat CD4 (Green in B). C is the merged image with nuclei counter-stained blue using DAPI. Low power.

GTX41868 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody [ED1].

GTX41868 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody [ED1].

GTX41868 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody (red n A) and Mouse anti Rat CD4 (Green in B). C is the merged image with nuclei counter-stained blue using DAPI.Medium power

GTX41868 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX41868 CD68 antibody (red n A) and Mouse anti Rat CD4 (Green in B). C is the merged image with nuclei counter-stained blue using DAPI. High power.