PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A moderate to strong, predominantly membranous staining of T-cells in the interfollicular zones and the germinal centres should be seen. Colon: A moderate to strong staining of the intra-epithelial T-cells in the colon mucosa should be seen.
Negative Control
Tonsil/Colon: All epithelial structures should stain negative.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for BSA and azide free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human CD7
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD7 molecule , GP40 , LEU-9 , TP41 , Tp40
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390](/templates/default/js/lightbox/images/loading.gif "Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390")
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GTX04390 IHC-P Image
IHC-P analysis of human colon tissue section using GTX04390 CD7 antibody [MSVA-007R] HistoMAX™.
Strong CD7 staining of a large fraction of tumor infiltrating lymphocytes in a colorectal adenocarcinoma. Tumor glands are completely CD7 negative.
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GTX04390 IHC-P Image
IHC-P analysis of human lung tissue section using GTX04390 CD7 antibody [MSVA-007R] HistoMAX™.
Strong membranous CD7 expression of an adenocarcinoma of the lung.
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GTX04390 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX04390 CD7 antibody [MSVA-007R] HistoMAX™.
Strong CD7 positivity of T lymphocytes mainly in the interfollicular area.
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REFERENCE
There are currently no references for CD7 antibody [MSVA-007R] HistoMAX™ (GTX04390). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD7 antibody [MSVA-007R] HistoMAX™ (GTX04390). Be the first to share your experience with this product.
![Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390](/upload/website/prouct_img/small/GTX04390/GTX04390_20230728_IHC-P_14_23072722_645.webp "Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390")
![Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390](/upload/website/prouct_img/small/GTX04390/GTX04390_20230728_IHC-P_144_23072722_653.webp "Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390")
![Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390](/upload/website/prouct_img/small/GTX04390/GTX04390_20230728_IHC-P_274_23072723_407.webp "Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390")
![Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390](/upload/website/prouct_img/normal/GTX04390/GTX04390_20230728_IHC-P_14_23072722_645.webp "Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390")
![Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390](/upload/website/prouct_img/normal/GTX04390/GTX04390_20230728_IHC-P_144_23072722_653.webp "Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390")
![Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390](/upload/website/prouct_img/normal/GTX04390/GTX04390_20230728_IHC-P_274_23072723_407.webp "Anti-CD7 antibody [MSVA-007R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04390")