GeneTex
United States (US)

CD71 antibody [MEM-75]

GTX29179_Transferrin-receptor-antibody-MEM-75__GTX29179-1_19012723_566.jpeg
Transferrin-receptor-antibody-MEM-75__GTX29179-1_18121411_716.png

Cat No. GTX29179

Host Mouse
Clonality Monoclonal
Clone Name MEM-75
Isotype IgG1
Application ICC/IF, FACS, IP
Reactivity Human
APPLICATION

Application Note

FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

85 kDa. ( Note )

Specificity/Sensitivity

Human CD71 (transferrin receptor). This antibody does not block the binding of transferrin to the receptor.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Pre-B cell line NALM-6.

Purification

>95% by SDS-PAGE
Purified by protein A affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transferrin Receptor,Cd71,Imd46,T9,Tfr,Tfr1,Tr,Trfr,P90,Tfrc

Background

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

Database

Research Area

DATA IMAGES
GTX29179_Transferrin-receptor-antibody-MEM-75__GTX29179-1_19012723_566.jpeg

GTX29179 FACS Image

Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75): Biotin (GTX78311); detection by Streptavidin-PE . Total viable cells were used for analysis.

Transferrin-receptor-antibody-MEM-75__GTX29179-1_18121411_716.png

GTX29179 ICC/IF Image

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75; green;GTX29179). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

REFERENCE