GeneTex
United States (US)

CD71 antibody [N2C1], Internal

KO/KD Comparison
GTX102596_42025_20181123_WB_KO_watermark_19011519_172.jpg
GTX102596_42025_20171110_WB_competitor_watermark_19011506_328.jpg
GTX102596_42025_20150629_WB_merged_19011506_751.jpg
GTX102596_42025_20150831_IFA_19011506_832.jpg

Cat No. GTX102596

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , K562 , HL-60 , NIH3T3

Predict Reactivity

Bovine, Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD71. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD71 antibody, TFR antibody, TFR1 antibody, TRFR antibody, TFRC antibody, transferrin receptor protein 1 antibody, "transferrin receptor (p90, CD71) antibody"

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Melanosome , Transferrin receptor protein 1 , serum form: Secreted

Background

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.

Database

Research Area

DATA IMAGES
GTX102596_42025_20181123_WB_KO_watermark_19011519_172.jpg

GTX102596 WB Image

KO/KD

Wild-type (WT) and CD71 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX102596_42025_20171110_WB_competitor_watermark_19011506_328.jpg

GTX102596 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500 and competitor's antibody (sc-32272) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX102596_42025_20150629_WB_merged_19011506_751.jpg

GTX102596 WB Image

CD71 antibody detects CD71 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody (GTX102596) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102596_42025_20150831_IFA_19011506_832.jpg

GTX102596 ICC/IF Image

CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD71 protein stained by CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf