*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Jurkat , Raji , NCI-H929 , K562 , HL-60 , NIH3T3
Bovine, Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human CD71. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cell membrane; Single-pass type II membrane protein , Melanosome , Transferrin receptor protein 1 , serum form: Secreted
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
GTX102596 WB Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500 and competitor's antibody (sc-32272) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
GTX102596 WB Image
CD71 antibody detects CD71 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody (GTX102596) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX102596 ICC/IF Image
CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD71 protein stained by CD71 antibody [N2C1], Internal (GTX102596) diluted at 1:500.
Blue: Hoechst 33342 staining.