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CD71 antibody [TFRC/1817]

Anti-CD71 antibody [TFRC/1817] used in IHC (Paraffin sections) (IHC-P). GTX34549
Anti-CD71 antibody [TFRC/1817] used in IHC (Paraffin sections) (IHC-P). GTX34549
Anti-CD71 antibody [TFRC/1817] used in Western Blot (WB). GTX34549

Cat No. GTX34549

Host

Mouse

Clonality

Monoclonal

Clone Name

TFRC/1817

Isotype

IgG2b

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA 2-4μg/ml (for coating)

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Specificity/Sensitivity

This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD71 , IMD46 , T9 , TFR , TFR1 , TFRC , TR , TRFR , p90 , transferrin receptor , Transferrin receptor 1 , mammary tumor virus receptor 1

Cellular Localization

Cell membrane,Melanosome,Secreted

Background

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-CD71 antibody [TFRC/1817] used in IHC (Paraffin sections) (IHC-P). GTX34549

GTX34549 IHC-P Image

IHC-P analysis of human bone marrow tissue using GTX34549 CD71 antibody [TFRC/1817].

Anti-CD71 antibody [TFRC/1817] used in IHC (Paraffin sections) (IHC-P). GTX34549

GTX34549 IHC-P Image

IHC-P analysis of human placenta tissue using GTX34549 CD71 antibody [TFRC/1817].

Anti-CD71 antibody [TFRC/1817] used in Western Blot (WB). GTX34549

GTX34549 WB Image

WB analysis of (A) Jurkat (B) HeLa (C) MCF-7 (D) K562 cell lysates using GTX34549 CD71 antibody [TFRC/1817].

REFERENCE

There are currently no references for CD71 antibody [TFRC/1817] (GTX34549). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD71 antibody [TFRC/1817] (GTX34549). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf