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CD71 antibody [TFRC/1817]

Anti-CD71 antibody [TFRC/1817] used in Western Blot (WB). GTX34549
Anti-CD71 antibody [TFRC/1817] used in IHC (Paraffin sections) (IHC-P). GTX34549

Cat No. GTX34549

Host Mouse
Clonality Monoclonal
Clone Name TFRC/1817
Isotype IgG2b
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Specificity/Sensitivity

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human TFRC protein fragment (aa94-212) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transferrin Receptor,Cd71,Imd46,T9,Tfr,Tfr1,Tr,Trfr,P90,Tfrc

Database

Research Area

DATA IMAGES
Anti-CD71 antibody [TFRC/1817] used in Western Blot (WB). GTX34549

GTX34549 WB Image

Western Blot Analysis of A) Jurkat (B) HeLa (C) MCF-7 & (D) K562 Cell lysate using CD71 Mouse Monoclonal Antibody (TFRC/1817).

Anti-CD71 antibody [TFRC/1817] used in IHC (Paraffin sections) (IHC-P). GTX34549

GTX34549 IHC-P Image

Formalin-fixed, paraffin-embedded Human Placenta stained with CD71 Monoclonal Antibody (TFRC/1817).