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CD71 antibody

Anti-CD71 antibody used in Immunoprecipitation (IP). GTX33080
Anti-CD71 antibody used in Western Blot (WB). GTX33080
Anti-CD71 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33080
Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080
Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080
Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080
Anti-CD71 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33080
Anti-CD71 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33080
Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080

Cat. No. GTX33080

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD71 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TFRC (NP_001121620.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transferrin receptor , CD71 , IMD46 , T9 , TFR , TFR1 , TR , TRFR , p90

Cellular Localization

Cell membrane

Background

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-CD71 antibody used in Immunoprecipitation (IP). GTX33080

GTX33080 IP Image

IP analysis of Jurkat cell lysate using GTX33080 CD71 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-CD71 antibody used in Western Blot (WB). GTX33080

GTX33080 WB Image

WB analysis of various sample lysates using GTX33080 CD71 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-CD71 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33080

GTX33080 ICC/IF Image

ICC/IF analysis of C6 cells using GTX33080 CD71 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080

GTX33080 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX33080 CD71 antibody.
Dilution : 1:100

Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080

GTX33080 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX33080 CD71 antibody.
Dilution : 1:100

Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080

GTX33080 IHC-P Image

IHC-P analysis of human placenta tissue using GTX33080 CD71 antibody.
Dilution : 1:100

Anti-CD71 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33080

GTX33080 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33080 CD71 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD71 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33080

GTX33080 ICC/IF Image

ICC/IF analysis of L929 cells using GTX33080 CD71 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD71 antibody used in IHC (Paraffin sections) (IHC-P). GTX33080

GTX33080 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX33080 CD71 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399