APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
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Note :
ICC/IF
HUVEC cells were seeded on gelatin-coated coverslips and stained with antibody.
IHC-Fr
Tissue sections were fixed in acetone.- As positive control anti-CD31 was used and as negative control an isotype antibody.
Neutralizing/Inhibition
Antibody 4G4 functions as an inhibitor of lymphocyte binding to HUVEC. The antibody was functionally tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer. Furthermore the monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes and reduces enzyme activity.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody stains the extracellular domain of CD73. The monoclonal antibody 4G4 recognizes both membrane bound and soluble human CD73, also known as ecto-5’-nucleotidase. The monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Inflamed synovial stroma from rheumatoid arthritis patients
Purification
Protein G purified
Endotoxin
< 24 EU/mg (Determined by LAL assay)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CALJA,CD73,E5NT,NT,NT5,NT5E,NTE,eN,eNT,5'nucleotidase ecto,5'-nucleotidase ecto
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Database
Research Area
REFERENCE
There are currently no references for CD73 antibody [4G4] (GTX54475). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD73 antibody [4G4] (GTX54475). Be the first to share your experience with this product.
