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CD73 antibody [HL2493]

Recombinant antibody KO/KD Orthogonal
Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851
Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851
Anti-CD73 antibody [HL2493] used in IHC (Paraffin sections) (IHC-P). GTX638851
Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851
Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851

Cat. No. GTX638851

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2493

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all CD73 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CD73.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5''-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT

Background

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851

GTX638851 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851

GTX638851 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [HL2493] used in IHC (Paraffin sections) (IHC-P). GTX638851

GTX638851 IHC-P Image

CD73 antibody [HL2493] detects CD73 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD73 stained by CD73 antibody [HL2493] (GTX638851) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851

GTX638851 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CD73 antibody [HL2493] used in Western Blot (WB). GTX638851

GTX638851 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [HL2493] (GTX638851) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations