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CD73 antibody [N3C3]

KO/KD Orthogonal
Anti-CD73 antibody [N3C3] used in Western Blot (WB). GTX101140
Anti-CD73 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101140
Anti-CD73 antibody [N3C3] used in Western Blot (WB). GTX101140
Anti-CD73 antibody [N3C3] used in Western Blot (WB). GTX101140

Cat. No. GTX101140

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD73 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951033

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT

Cellular Localization

Cell membrane , GPI-anchor

Background

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CD73 antibody [N3C3] used in Western Blot (WB). GTX101140

GTX101140 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N3C3] (GTX101140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101140

GTX101140 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB231 xenograft, using CD73(GTX101140) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD73 antibody [N3C3] used in Western Blot (WB). GTX101140

GTX101140 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX101140 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD73 antibody [N3C3] used in Western Blot (WB). GTX101140

GTX101140 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2015.PMID: 26273418

REFERENCE

Application Reference

Ritter A et al. Cells 2019; 8 (10) Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity.
Application : ICC/IF
Reactivity : Human
Maraldi T et al. Oxid Med Cell Longev 2015; 2015 : 101304 Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells.
Application : WB
Reactivity : Human
Resca E et al. Placenta 2015; 36 (1) : 18-26 Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169