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CD73 antibody

KO/KD
Anti-CD73 antibody used in IHC (Paraffin sections) (IHC-P). GTX54599
Anti-CD73 antibody used in Western Blot (WB). GTX54599
Anti-CD73 antibody used in IHC (Paraffin sections) (IHC-P). GTX54599
Anti-CD73 antibody used in Western Blot (WB). GTX54599

Cat. No. GTX54599

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD73 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E (NP_002517.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5'-nucleotidase ecto , CALJA , CD73 , E5NT , NT , NT5 , NTE , eN , eNT

Background

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-CD73 antibody used in IHC (Paraffin sections) (IHC-P). GTX54599

GTX54599 IHC-P Image

IHC-P analysis of human placenta tissue using GTX54599 CD73 antibody.
Dilution : 1:200

Anti-CD73 antibody used in Western Blot (WB). GTX54599

GTX54599 WB Image

WB analysis of various sample lysates using GTX54599 CD73 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD73 antibody used in IHC (Paraffin sections) (IHC-P). GTX54599

GTX54599 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX54599 CD73 antibody.
Dilution : 1:200

Anti-CD73 antibody used in Western Blot (WB). GTX54599

GTX54599 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HCT116 cell lysate using GTX54599 CD73 antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399