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CD74 antibody [CLIP/3127R]

Anti-CD74 antibody [CLIP/3127R] used in IHC (Paraffin sections) (IHC-P). GTX02612
Anti-CD74 antibody [CLIP/3127R] used in Flow cytometry (FACS). GTX02612
Anti-CD74 antibody [CLIP/3127R] used in Western Blot (WB). GTX02612

Cat. No. GTX02612

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 CLIP/3127R

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Baboon
Package
100 μg ($529)
See all CD74 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Observed MW

~40 kDa. The observed M.W. is based on the following publication. PMID: 29490700

Product Note

We do not recommend use of this product for Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SU-DHL-4 lymphoma cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD74 molecule , DHLAG , HLADG , II , Ia-GAMMA , p33

Cellular Localization

Cell membrane , Endoplasmic reticulum membrane , Endosome , Lysosome

Background

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD74 antibody [CLIP/3127R] used in IHC (Paraffin sections) (IHC-P). GTX02612

GTX02612 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02612 CD74 antibody [CLIP/3127R].

Anti-CD74 antibody [CLIP/3127R] used in Flow cytometry (FACS). GTX02612

GTX02612 FACS Image

FACS analysis of Raji cells using GTX02612 CD74 antibody [CLIP/3127R].
Blue : Primary antibody
Red : Isotype control

Anti-CD74 antibody [CLIP/3127R] used in Western Blot (WB). GTX02612

GTX02612 WB Image

WB analysis of 293T whole cell lysate using GTX02612 CD74 antibody [CLIP/3127R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529