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CD74 antibody [CLIP/813]

Anti-CD74 antibody [CLIP/813] used in IHC (Paraffin sections) (IHC-P). GTX34553
Anti-CD74 antibody [CLIP/813] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34553
Anti-CD74 antibody [CLIP/813] used in Flow cytometry (FACS). GTX34553

Cat No. GTX34553

Host

Mouse

Clonality

Monoclonal

Clone Name

CLIP/813

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

ICC/IF
Methanol Fixed

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human CD74 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD74 , CD74 molecule , DHLAG , HLADG , II , IaGAMMA

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus,Trans-Golgi network,Endosome,Lysosome

Background

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD74 antibody [CLIP/813] used in IHC (Paraffin sections) (IHC-P). GTX34553

GTX34553 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34553 CD74 antibody [CLIP/813].

Anti-CD74 antibody [CLIP/813] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34553

GTX34553 ICC/IF Image

ICC/IF analysis of Raji cells using GTX34553 CD74 antibody [CLIP/813].
Green : Primary antibody
Red : nucleus

Anti-CD74 antibody [CLIP/813] used in Flow cytometry (FACS). GTX34553

GTX34553 FACS Image

FACS analysis of Raji cells using GTX34553 CD74 antibody [CLIP/813].
Blue : Primary antibody
Red : Isotype control

REFERENCE

There are currently no references for CD74 antibody [CLIP/813] (GTX34553). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD74 antibody [CLIP/813] (GTX34553). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339