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CD74 antibody [N1N2], N-term

Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477
Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477
Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477
Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477

Cat No. GTX110477

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Raji , GL261 , Rat2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CD74. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949933

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD74 molecule , DHLAG , HLADG , II , Ia-GAMMA , p33

Cellular Localization

Membrane; Single-pass type II membrane protein

Database

Research Area

DATA IMAGES

Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477

GTX110477 IHC-P Image

CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477

GTX110477 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477

GTX110477 WB Image

CD74 antibody [N1N2], N-term detects CD74 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
12% SDS-PAGE
CD74 antibody [N1N2], N-term (GTX110477) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477

GTX110477 WB Image

CD74 antibody [N1N2], N-term detects CD74 protein by western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
12% SDS-PAGE
CD74 antibody [N1N2], N-term (GTX110477) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

CD74 antibody [N1N2], N-term Cat No. GTX110477

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: HEK293 transfected with vector
Lane 2: HEK293 transfected with CD74 variant 1
Lane 3: HEK293 transfected with CD74 variant 2
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Kidney tissue
Fixation : Paraffin-embedded
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.5Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Mouse kidney, 16 week.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169