Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

CD74 antibody [N1N2], N-term detects CD74 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.