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CD74 antibody [N1N2], N-term

Overexpression
Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477
Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477
Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477
Anti-CD74 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110477
Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477
Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477

Cat. No. GTX110477

Reference 4
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD74 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CD74. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949933

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD74 molecule , DHLAG , HLADG , II , Ia-GAMMA , p33

Cellular Localization

Membrane; Single-pass type II membrane protein

Database

Research Area

DATA IMAGES

Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477

GTX110477 IHC-P Image

CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477

GTX110477 IHC-P Image

CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477

GTX110477 IHC-P Image

CD74 antibody [N1N2], N-term detects CD74 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD74 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110477

GTX110477 ICC/IF Image

CD74 antibody [N1N2], N-term detects CD74 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD74 stained by CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477

GTX110477 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477

GTX110477 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD74 antibody [N1N2], N-term (GTX110477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sreevalsan S et al. EMBO Rep 2020; 21 (12) : e50155 MLLT6 maintains PD-L1 expression and mediates tumor immune resistance.
Liu YH et al. Biosci Rep 2020; 40 (8) Association of variant on the promoter of cluster of differentiation 74 in graves disease and graves ophthalmopathy.
Application : WB
Guest JD et al. Biomaterials 2018; 185 : 284-300 Internal decompression of the acutely contused spinal cord: Differential effects of irrigation only versus biodegradable scaffold implantation.
Application : IHC-P
Reactivity : Rat
Chen Y-T et al. J Pineal Res 2014; 57 (3) : 248-61 Melatonin Treatment further Improves Adipose-Derived Mesenchymal Stem Cell Therapy for Acute Interstitial Cystitis in Rat.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

CD74 antibody [N1N2], N-term Cat. No. GTX110477

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-CD74 antibody [N1N2], N-term used in Western Blot (WB). GTX110477
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 50 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: HEK293 transfected with vector
Lane 2: HEK293 transfected with CD74 variant 1
Lane 3: HEK293 transfected with CD74 variant 2
Anti-CD74 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX110477
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Kidney tissue
Fixation : Paraffin-embedded
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.5Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Mouse kidney, 16 week.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169