GeneTex
United States (US)

CD74 antibody [SPM523]

GTX34552_20170712_IHC-P_18121410_210.jpg

Cat No. GTX34552

Host Mouse
Clonality Monoclonal
Clone Name SPM523
Isotype IgG1
Application IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

IHC-P:1-2ug/ml for 30 minutes at RT.

Calculated MW

34 kDa. ( Note )

Specificity/Sensitivity

CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

SU-DHL-4 lymphoma cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd74 Molecule,Dhlag,Hladg,Ii,Ia-Gamma,P33,Cd74

Background

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX34552_20170712_IHC-P_18121410_210.jpg

GTX34552 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Ab (SPM523).

Package List Price ($)
$ 329