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CD74 antibody

Anti-CD74 antibody used in IHC (Paraffin sections) (IHC-P). GTX02203
Anti-CD74 antibody used in IHC (Paraffin sections) (IHC-P). GTX02203
Anti-CD74 antibody used in IHC (Paraffin sections) (IHC-P). GTX02203
Anti-CD74 antibody used in Western Blot (WB). GTX02203
Anti-CD74 antibody used in Immunoprecipitation (IP). GTX02203

Cat No. GTX02203

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:500-1:2000
IP 0.5-2 μl/mg of lysate
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human CD74 protein

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD74 molecule , DHLAG , HLADG , II , Ia-GAMMA , p33

Cellular Localization

Cell membrane , Endoplasmic reticulum membrane , Endosome , Lysosome

Background

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD74 antibody used in IHC (Paraffin sections) (IHC-P). GTX02203

GTX02203 IHC-P Image

IHC-P analysis of human lung tissue section using GTX02203 CD74 antibody.
Dilution : 1:1000

Anti-CD74 antibody used in IHC (Paraffin sections) (IHC-P). GTX02203

GTX02203 IHC-P Image

IHC-P analysis of human colon carcinoma tissue section using GTX02203 CD74 antibody.
Dilution : 1:1000

Anti-CD74 antibody used in IHC (Paraffin sections) (IHC-P). GTX02203

GTX02203 IHC-P Image

IHC-P analysis of human rectal cancer tissue section using GTX02203 CD74 antibody.
Dilution : 1:1000

Anti-CD74 antibody used in Western Blot (WB). GTX02203

GTX02203 WB Image

WB analysis of Raji whole cell lysate (Lane A) using GTX02203 CD74 antibody.
Dilution : 1:500
Loading : 30 μg

Anti-CD74 antibody used in Immunoprecipitation (IP). GTX02203

GTX02203 IP Image

IP analysis of HuT78 whole cell lysate using GTX02203 CD74 antibody.
IP antibody : 2 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
50 % Protein G agarose : 15 μl

REFERENCE

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REVIEW

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Package List Price ($)
$ 339