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CD79a antibody [SPM550]

Anti-CD79a antibody [SPM550] used in IHC (Paraffin sections) (IHC-P). GTX34558
Anti-CD79a antibody [SPM550] used in Western Blot (WB). GTX34558
Anti-CD79a antibody [SPM550] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34558

Cat No. GTX34558

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM550

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Bovine, Pig, Monkey
Package
100 μg ($339)
See all CD79a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD79A , CD79a molecule , IGA , MB1 , CD79a

Cellular Localization

Cell membrane

Background

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD79a antibody [SPM550] used in IHC (Paraffin sections) (IHC-P). GTX34558

GTX34558 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34558 CD79a antibody [SPM550].

Anti-CD79a antibody [SPM550] used in Western Blot (WB). GTX34558

GTX34558 WB Image

WB analysis of Raji cell lysate using GTX34558 CD79a antibody [SPM550].

Anti-CD79a antibody [SPM550] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34558

GTX34558 ICC/IF Image

ICC/IF analysis of PFA-fixed Raji cells using GTX34558 CD79a antibody [SPM550].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339