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CD8 alpha antibody [53-6.7]

Cat. No. GTX00604

Reference 11

Host

 Rat

Clonality

 Monoclonal

Clone Name

 53-6.7

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, IP, Depletion

Reactivity

 Mouse
Package
100 μg ($399)
See all CD8 alpha products
PRODUCT

Summary

The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Depletion Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse spleen cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD8 antigen, alpha chain , BB154331 , Ly-2 , Ly-35 , Ly-B , Lyt-2

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Clone Reference

2019; Ex vivo Hsp70-Activated NK Cells in Combination With PD-1 Inhibition Significantly Increase Overall Survival in Preclinical Models of Glioblastoma and Lung Cancer
Application : IHC-P
Reactivity : Mouse
Hsieh JL et al. Int J Mol Sci 2013; 14 (10):19951-70 CD8+ T cell-induced expression of tissue inhibitor of metalloproteinses-1 exacerbated osteoarthritis.
2012; Activation of the Hippo pathway by CTLA-4 regulates the expression of Blimp-1 in the CD8+ T cell
Application : IHC-Fr
Reactivity : Mouse
2012; Canonical autophagy dependent on the class III phosphoinositide-3 kinase Vps34 is required for naive T-cell homeostasis
Application : FACS
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
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REVIEW

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Clone Reference
2019; Ex vivo Hsp70-Activated NK Cells in Combination With PD-1 Inhibition Significantly Increase Overall Survival in Preclinical Models of Glioblastoma and Lung Cancer
Application : IHC-P
Reactivity : Mouse
Hsieh JL et al. Int J Mol Sci 2013; 14 (10):19951-70 CD8+ T cell-induced expression of tissue inhibitor of metalloproteinses-1 exacerbated osteoarthritis.
2012; Activation of the Hippo pathway by CTLA-4 regulates the expression of Blimp-1 in the CD8+ T cell
Application : IHC-Fr
Reactivity : Mouse
2012; Canonical autophagy dependent on the class III phosphoinositide-3 kinase Vps34 is required for naive T-cell homeostasis
Application : FACS
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
2008; Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2
Application : Depletion
Reactivity : Mouse
2006; CD4+ regulatory T cells are spared from deletion by antilymphocyte serum, a polyclonal anti-T cell antibody
Application : ICC/IF
2005; Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
Application : Depletion
2005; Sodium stibogluconate interacts with IL-2 in anti-Renca tumor action via a T cell-dependent mechanism in connection with induction of tumor-infiltrating macrophages
Application : IHC-Fr
Reactivity : Mouse
2002; Vaccination with minigenes encoding V(H)-derived major histocompatibility complex class I-binding epitopes activates cytotoxic T cells that ablate autoantibody-producing B cells and inhibit lupus
Application : Depletion
1999; Association of the Adaptor Molecule Lat with Cd4 and Cd8 Coreceptors Identifies a New Coreceptor Function in T Cell Receptor Signal Transduction
Application : FACS, IP
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399