Immunofluorescence 5-20 ug/mL. Flow cytometry 1-5 ug/10^6 cells. Immunohistochemistry 5-20 ug/mL on frozen sections using avidin-biotin system. The optimal dilution factors should be determined by the individual laboratory. This antibody was not quality controlled in flow cytometry. This antibody can be used on frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope.
Tonsil and Lymph Node
This antibody reacts with a 32 kD molecule present on human suppressor/cytotoxic T-cells. These antibodies also react with sinus-lining cells in the human spleen.
Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
E-rosette forming cells purified from human PBL
Protein A affinity purified
From hybridoma culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.