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CD80 antibody [1E9]

Overexpression
Anti-CD80 antibody [1E9] used in Flow cytometry (FACS). GTX84698
Anti-CD80 antibody [1E9] used in Western Blot (WB). GTX84698
Anti-CD80 antibody [1E9] used in Western Blot (WB). GTX84698
Anti-CD80 antibody [1E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84698

Cat. No. GTX84698

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E9

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all CD80 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD80 molecule , B7 , B7-1 , B7.1 , BB1 , CD28LG , CD28LG1 , LAB7

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Database

Research Area

DATA IMAGES

Anti-CD80 antibody [1E9] used in Flow cytometry (FACS). GTX84698

GTX84698 FACS Image

FACS analysis of HEK293T cells transfected with either CD80 plasmid(Red) or empty vector control plasmid(Blue) using GTX84698 CD80 antibody [1E9].

Anti-CD80 antibody [1E9] used in Western Blot (WB). GTX84698

GTX84698 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CD80 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84698 CD80 antibody [1E9].
Loading : 5 ug per lane

Anti-CD80 antibody [1E9] used in Western Blot (WB). GTX84698

GTX84698 WB Image

WB analysis of various cell lines using GTX84698 CD80 antibody [1E9].
Loading : 35 ug per lane

Anti-CD80 antibody [1E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84698

GTX84698 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD80 plasmid using GTX84698 CD80 antibody [1E9].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519