GeneTex
United States (US)

CD80 antibody [2B11]

GTX84701_3044_IHC-P_19021902_874.jpg
GTX84701_3051_IHC-P_19021902_303.jpg
GTX84701_4573_WB_18122101_962.jpg
GTX84701_1198_ICCIF_18122101_821.jpg
GTX84701_3045_IHC-P_19021902_350.jpg
GTX84701_3049_IHC-P_19021902_602.jpg
GTX84701_3043_IHC-P_19021902_948.jpg
GTX84701_3048_IHC-P_19021902_178.jpg
GTX84701_3052_IHC-P_19021902_403.jpg
GTX84701_3046_IHC-P_19021902_906.jpg
GTX84701_4210_WB_19021902_482.jpg
GTX84701_3042_IHC-P_19021902_238.jpg
GTX84701_3047_IHC-P_19021902_650.jpg
GTX84701_3050_IHC-P_19021902_516.jpg

Cat No. GTX84701

Host Mouse
Clonality Monoclonal
Clone Name 2B11
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
Not tested in other applications.

Calculated MW

33 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd80 Molecule,B7,B7-1,B7.1,Bb1,Cd28Lg,Cd28Lg1,Lab7,Cd80

Cellular Localization

Membrane

Background

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Database

Research Area

DATA IMAGES
GTX84701_3044_IHC-P_19021902_874.jpg

GTX84701 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3051_IHC-P_19021902_303.jpg

GTX84701 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_4573_WB_18122101_962.jpg

GTX84701 WB Image

WB analysis of HEK293T cells transfected with CD80 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84701 CD80 antibody [2B11].
Loading : 5 ug per lane

GTX84701_1198_ICCIF_18122101_821.jpg

GTX84701 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD80 plasmid using GTX84701 CD80 antibody [2B11].

GTX84701_3045_IHC-P_19021902_350.jpg

GTX84701 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3049_IHC-P_19021902_602.jpg

GTX84701 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3043_IHC-P_19021902_948.jpg

GTX84701 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3048_IHC-P_19021902_178.jpg

GTX84701 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3052_IHC-P_19021902_403.jpg

GTX84701 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3046_IHC-P_19021902_906.jpg

GTX84701 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_4210_WB_19021902_482.jpg

GTX84701 WB Image

WB analysis of HepG2 cell lysate using GTX84701 CD80 antibody [2B11].
Loading : 10 ug per lane
Dilution : 1:200

GTX84701_3042_IHC-P_19021902_238.jpg

GTX84701 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3047_IHC-P_19021902_650.jpg

GTX84701 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

GTX84701_3050_IHC-P_19021902_516.jpg

GTX84701 IHC-P Image

IHC-P analysis of human liver tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.