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CD80 antibody [2B11]

Anti-CD80 antibody [2B11] used in Western Blot (WB). GTX84701
Anti-CD80 antibody [2B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in Western Blot (WB). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701
Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

Cat No. GTX84701

Host Mouse
Clonality Monoclonal
Clone Name 2B11
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($379)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
Not tested in other applications.

Calculated MW

33 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd80 Molecule,B7,B7-1,B7.1,Bb1,Cd28Lg,Cd28Lg1,Lab7,Cd80

Cellular Localization

Membrane

Background

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Database

Research Area

DATA IMAGES
Anti-CD80 antibody [2B11] used in Western Blot (WB). GTX84701

GTX84701 WB Image

WB analysis of HEK293T cells transfected with CD80 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84701 CD80 antibody [2B11].
Loading : 5 ug per lane

Anti-CD80 antibody [2B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84701

GTX84701 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD80 plasmid using GTX84701 CD80 antibody [2B11].

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in Western Blot (WB). GTX84701

GTX84701 WB Image

WB analysis of HepG2 cell lysate using GTX84701 CD80 antibody [2B11].
Loading : 10 ug per lane
Dilution : 1:200

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

Anti-CD80 antibody [2B11] used in IHC (Paraffin sections) (IHC-P). GTX84701

GTX84701 IHC-P Image

IHC-P analysis of human liver tissue using GTX84701 CD80 antibody [2B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.

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Package List Price ($)
$ 379