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CD80 antibody [2E5]

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700
Anti-CD80 antibody [2E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84700
Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700
Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700
Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700
Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700
Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700
Anti-CD80 antibody [2E5] used in Western Blot (WB). GTX84700
Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

Cat. No. GTX84700

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2E5

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($539)
See all CD80 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD80 molecule , B7 , B7-1 , B7.1 , BB1 , CD28LG , CD28LG1 , LAB7

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Database

Research Area

DATA IMAGES

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

GTX84700 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD80 antibody [2E5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84700

GTX84700 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD80 plasmid using GTX84700 CD80 antibody [2E5].

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

GTX84700 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

GTX84700 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

GTX84700 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

GTX84700 IHC-P Image

IHC-P analysis of human liver tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

GTX84700 IHC-P Image

IHC-P analysis of human colon tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD80 antibody [2E5] used in Western Blot (WB). GTX84700

GTX84700 WB Image

WB analysis of HEK293T cells transfected with CD80 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84700 CD80 antibody [2E5].
Loading : 5 ug per lane

Anti-CD80 antibody [2E5] used in IHC (Paraffin sections) (IHC-P). GTX84700

GTX84700 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539