CD80 antibody [2E5]

Cat. No. GTX84700

Host	Mouse
Clonality	Monoclonal
Clone Name	2E5
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($539)

See all CD80 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:150

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD80 molecule , B7 , B7-1 , B7.1 , BB1 , CD28LG , CD28LG1 , LAB7

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Database

Gene ID: 941 CD80
UniProt: P33681 CD80

Research Area

DATA IMAGES

GTX84700 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD80 plasmid using GTX84700 CD80 antibody [2E5].

GTX84700 WB Image

WB analysis of HEK293T cells transfected with CD80 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84700 CD80 antibody [2E5].
Loading : 5 ug per lane

GTX84700 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84700 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84700 IHC-P Image

IHC-P analysis of human liver tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84700 IHC-P Image

IHC-P analysis of human colon tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84700 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84700 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84700 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84700 CD80 antibody [2E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 539

Qty

CD80 antibody [2E5]

Cat. No. GTX84700

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84700 ICC/IF Image

GTX84700 WB Image

GTX84700 IHC-P Image

GTX84700 IHC-P Image

GTX84700 IHC-P Image

GTX84700 IHC-P Image

GTX84700 IHC-P Image

GTX84700 IHC-P Image

GTX84700 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.