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CD80 antibody [MEM-233]

Cat. No. GTX75761

Reference 18

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-233

Isotype

 IgG1

Application

 WB, FACS, IP

Reactivity

 Human, Monkey
Package
200 μg ($459)
See all CD80 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

This antibody binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching studies (Vasu et al. 2003).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_377015

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD80 molecule , B7 , B7-1 , B7.1 , BB1 , CD28LG , CD28LG1 , LAB7

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Database

Research Area

REFERENCE

Application Reference

Manferdini C et al. Biology (Basel) 2020; 9 (12) Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
Paolella F et al. J Cell Physiol 2018; (Epub) Effect of microfragmented adipose tissue on osteoarthritic synovial macrophage factors.
Application : FACS
Reactivity : Human
Manferdini C et al. Arthritis Res Ther 2016; 18 (1) : 83 From osteoarthritic synovium to synovial-derived cells characterization: synovial macrophages are key effector cells.
Application : FACS
Reactivity : Human

Clone Reference

Hovden AO et al. PLoS One 2012; 7 (2):e31217 The bacterial preparation OK432 induces IL-12p70 secretion in human dendritic cells in a TLR3 dependent manner.
Application : FACS
Reactivity : Human
Silk KM et al. Gene Ther 2012; 19 (10):1035-40 Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
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REVIEW

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Application Reference
Manferdini C et al. Biology (Basel) 2020; 9 (12) Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
Paolella F et al. J Cell Physiol 2018; (Epub) Effect of microfragmented adipose tissue on osteoarthritic synovial macrophage factors.
Application : FACS
Reactivity : Human
Manferdini C et al. Arthritis Res Ther 2016; 18 (1):83 From osteoarthritic synovium to synovial-derived cells characterization: synovial macrophages are key effector cells.
Application : FACS
Reactivity : Human
Clone Reference
Silk KM et al. J Biomed Biotechnol 2012; 2012:172420 Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
Application : FACS
Reactivity : Human
Hovden AO et al. PLoS One 2012; 7 (2):e31217 The bacterial preparation OK432 induces IL-12p70 secretion in human dendritic cells in a TLR3 dependent manner.
Application : FACS
Reactivity : Human
Silk KM et al. Gene Ther 2012; 19 (10):1035-40 Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
Hovden AO et al. BMC Immunol 2011; 12:2 Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
Piconi S et al. J Immunol 2010; 185 (12):7723-30 Immunological effects of sublingual immunotherapy: clinical efficacy is associated with modulation of programmed cell death ligand 1, IL-10, and IgG4.
Trojan J et al. Biomed Pharmacother 2010; 64 (8):576-8 Antisense anti IGF-I cellular therapy of malignant tumours: immune response in cancer patients.
John J et al. BMC Immunol 2010; 11:14 differential effects of * on dendritic cell function.
Application : FACS
Daubenberger CA et al. Vet Immunol Immunopathol 2007; 119 (43102):14-20 Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of Aotus nancymaae, a non-human primate model for biomedical research.
Application : FACS
Reactivity : Monkey
Newman KC et al. PLoS Pathog 2006; 2 (12):e118 cross-talk with myeloid accessory cells regulates human natural killer cell *-gamma responses to malaria.
Application : FACS
Angel CE et al. J Immunol 2006; 176 (10):1398971 Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
Application : FACS
Tan PH et al. Blood 2005; 105 (10):3824-32 Modulation of human dendritic-cell function following transduction with viral vectors: implications for gene therapy.
Application : FACS
Huxley P et al. Chem Biol 2004; 11 (12):1651-8 High-affinity small molecule inhibitors of T cell costimulation: compounds for immunotherapy.
Tan PH et al. Atherosclerosis 2004; 173 (2):171-83 Phenotypic and functional differences between human saphenous vein (HSVEC) and umbilical vein (HUVEC) endothelial cells.
Zhan H et al. Invest Ophthalmol Vis Sci 2003; 44 (9):732951 The immunomodulatory role of human conjunctival epithelial cells.
Application : FACS
Scheinecker C et al. J Immunol 1998; 161 (8):3966-73 Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
Application : FACS
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459