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CD80 antibody [MEM-233]

Cat. No. GTX75761

Reference 18

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-233

Isotype

 IgG1

Application

 WB, FACS, IP

Reactivity

 Human, Monkey
Package
200 μg ($459)
See all CD80 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

This antibody binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching studies (Vasu et al. 2003).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_377015

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD80 molecule , B7 , B7-1 , B7.1 , BB1 , CD28LG , CD28LG1 , LAB7

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Database

Research Area

REFERENCE

Application Reference

Manferdini C et al. Biology (Basel) 2020; 9 (12) Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
Paolella F et al. J Cell Physiol 2018; (Epub) Effect of microfragmented adipose tissue on osteoarthritic synovial macrophage factors.
Application : FACS
Reactivity : Human
Manferdini C et al. Arthritis Res Ther 2016; 18 (1) : 83 From osteoarthritic synovium to synovial-derived cells characterization: synovial macrophages are key effector cells.
Application : FACS
Reactivity : Human

Clone Reference

Hovden AO et al. PLoS One 2012; 7 (2):e31217 The bacterial preparation OK432 induces IL-12p70 secretion in human dendritic cells in a TLR3 dependent manner.
Application : FACS
Reactivity : Human
Silk KM et al. Gene Ther 2012; 19 (10):1035-40 Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
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REVIEW

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Application Reference
Manferdini C et al. Biology (Basel) 2020; 9 (12) Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
Paolella F et al. J Cell Physiol 2018; (Epub) Effect of microfragmented adipose tissue on osteoarthritic synovial macrophage factors.
Application : FACS
Reactivity : Human
Manferdini C et al. Arthritis Res Ther 2016; 18 (1):83 From osteoarthritic synovium to synovial-derived cells characterization: synovial macrophages are key effector cells.
Application : FACS
Reactivity : Human
Clone Reference
Silk KM et al. J Biomed Biotechnol 2012; 2012:172420 Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
Application : FACS
Reactivity : Human
Hovden AO et al. PLoS One 2012; 7 (2):e31217 The bacterial preparation OK432 induces IL-12p70 secretion in human dendritic cells in a TLR3 dependent manner.
Application : FACS
Reactivity : Human
Silk KM et al. Gene Ther 2012; 19 (10):1035-40 Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
Hovden AO et al. BMC Immunol 2011; 12:2 Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
Piconi S et al. J Immunol 2010; 185 (12):7723-30 Immunological effects of sublingual immunotherapy: clinical efficacy is associated with modulation of programmed cell death ligand 1, IL-10, and IgG4.
Trojan J et al. Biomed Pharmacother 2010; 64 (8):576-8 Antisense anti IGF-I cellular therapy of malignant tumours: immune response in cancer patients.
John J et al. BMC Immunol 2010; 11:14 differential effects of * on dendritic cell function.
Application : FACS
Daubenberger CA et al. Vet Immunol Immunopathol 2007; 119 (43102):14-20 Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of Aotus nancymaae, a non-human primate model for biomedical research.
Application : FACS
Reactivity : Monkey
Newman KC et al. PLoS Pathog 2006; 2 (12):e118 cross-talk with myeloid accessory cells regulates human natural killer cell *-gamma responses to malaria.
Application : FACS
Angel CE et al. J Immunol 2006; 176 (10):1398971 Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
Application : FACS
Tan PH et al. Blood 2005; 105 (10):3824-32 Modulation of human dendritic-cell function following transduction with viral vectors: implications for gene therapy.
Application : FACS
Huxley P et al. Chem Biol 2004; 11 (12):1651-8 High-affinity small molecule inhibitors of T cell costimulation: compounds for immunotherapy.
Tan PH et al. Atherosclerosis 2004; 173 (2):171-83 Phenotypic and functional differences between human saphenous vein (HSVEC) and umbilical vein (HUVEC) endothelial cells.
Zhan H et al. Invest Ophthalmol Vis Sci 2003; 44 (9):732951 The immunomodulatory role of human conjunctival epithelial cells.
Application : FACS
Scheinecker C et al. J Immunol 1998; 161 (8):3966-73 Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
Application : FACS
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459