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CD81 antibody [1D6] (Low endotoxin, azide free)

Anti-CD81 antibody [1D6] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75436

Cat. No. GTX75436

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, Activation, Neutralizing/Inhibition

Reactivity

 Human, Goat, Sheep, Chimpanzee
Package
100 μg ($399)
See all CD81 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS 1/10
IP Assay dependent
Activation Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

OCI-LY8 cells aggregated by CD81 antibody[5A6]

Purification

Protein A purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_376799

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-CD81 antibody [1D6] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX75436

GTX75436 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX75436 CD81 antibody [1D6] (Low endotoxin, azide free).

REFERENCE

Application Reference

A N?meth et al. Cell Commun Signal 2024; 22 (1) : 282 Extracellular vesicles promote migration despite BRAF inhibitor treatment in malignant melanoma cells
Afrodit? N?meth et al. Research Square 2023; Extracellular vesicles promote migration despite vemurafenib treatment in malignant melanoma cells
Application : ICC/IF

Clone Reference

Kateryna Nesteruk et al. Clin Transl Gastroenterol 2022; 13 (3):e00465 Size and Concentration of Extracellular Vesicles in Pancreatic Juice From Patients With Pancreatic Ductal Adenocarcinoma.
Stella Chin-Shaw Tsai et al. Int J Mol Sci 2021; 22 (13)
Clerici SP et al. Front Cell Dev Biol 2021; 9:696373 Colorectal Cancer Cell-Derived Small Extracellular Vesicles Educate Human Fibroblasts to Stimulate Migratory Capacity.
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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations