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CD81 antibody [HL1666]

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264
Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264
Anti-CD81 antibody [HL1666] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637264
Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

Cat. No. GTX637264

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1666

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

Whole cell extract (1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

Jurkat membrane extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD81 antibody [HL1666] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637264

GTX637264 ICC/IF Image

CD81 antibody [HL1666] detects CD81 protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD81 stained by CD81 antibody [HL1666] (GTX637264) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD81 antibody [HL1666] used in Western Blot (WB). GTX637264

GTX637264 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody [HL1666] (GTX637264) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for CD81 antibody [HL1666] (GTX637264). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD81 antibody [HL1666] (GTX637264). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations