APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human CD81. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2909840
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Database
Research Area
DATA IMAGES
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GTX135297 IHC-P Image
CD81 antibody detects CD81 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.CD81 stained by CD81 antibody (GTX135297) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX135297 ICC/IF Image
CD81 antibody detects CD81 protein by immunofluorescent analysis.Sample: Jurkat and HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD81 stained by CD81 antibody (GTX135297) diluted at 1:500.
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GTX135297 WB Image
AsPC-1 whole cell extracts (5.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX135297) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX135297 IHC-P Image
CD81 antibody detects CD81 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse lymph node.CD81 stained by CD81 antibody (GTX135297) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX135297 ICC/IF Image
CD81 antibody detects CD81 protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD81 stained by CD81 antibody (GTX135297) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
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REVIEW
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