CD81 antibody

Cat. No. GTX31381

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μg ($399)

See all CD81 products

View product citations for antibody GTX31381 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	2.5 μg/mL
ICC/IF	Assay dependent
IHC-P	2 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

A549 Cell Slide(GTX25527) , RAW 264.7 Cell Lysate(GTX27187) , Human Lung Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81.The immunogen is located within amino acids 30 - 80 of CD81.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887616

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

Gene ID: 975 CD81
UniProt: P60033 CD81

Research Area

DATA IMAGES

GTX31381 IHC-P Image

IHC-P analysis of human lung tissue using GTX31381 CD81 antibody.
Working concentration : 5 μg/ml

GTX31381 IHC-P Image

IHC-P analysis of human brain tissue using GTX31381 CD81 antibody.
Working concentration : 10 μg/ml

GTX31381 WB Image

WB analysis of human lung tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31381 CD81 antibody.
Working concentration : 1 μg/ml