GeneTex
United States (US)

CD81 antibody

GTX31381_2581_WB_20180221_18121410_297.jpg
GTX31381_979_IHC-P_20180221_18121410_294.jpg
GTX31381_980_IHC-P_20180221_18121410_924.jpg
GTX31381_981_IHC-P_20180221_18121410_513.jpg
GTX31381_149_ICC-IF_20180221_18121410_534.jpg

Cat No. GTX31381

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2.5 μg/mL
ICC/IF Assay dependent
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

A549 Cell Slide(GTX25527) , RAW 264.7 Cell Lysate(GTX27187) , Human Lung Tissue Slide
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81.The immunogen is located within amino acids 30 - 80 of CD81.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd81 Molecule,Cvid6,S5.7,Tapa1,Tspan28,Cd81

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

DATA IMAGES
GTX31381_2581_WB_20180221_18121410_297.jpg

GTX31381 WB Image

WB analysis of human lung tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31381 CD81 antibody.
Working concentration : 1 μg/ml

GTX31381_979_IHC-P_20180221_18121410_294.jpg

GTX31381 IHC-P Image

IHC-P analysis of human brain tissue using GTX31381 CD81 antibody.
Working concentration : 10 μg/ml

GTX31381_980_IHC-P_20180221_18121410_924.jpg

GTX31381 IHC-P Image

IHC-P analysis of human brain tissue using GTX31381 CD81 antibody.
Working concentration : 2 μg/ml

GTX31381_981_IHC-P_20180221_18121410_513.jpg

GTX31381 IHC-P Image

IHC-P analysis of human lung tissue using GTX31381 CD81 antibody.
Working concentration : 5 μg/ml

GTX31381_149_ICC-IF_20180221_18121410_534.jpg

GTX31381 ICC/IF Image

ICC/IF analysis of A549 cells using GTX31381 CD81 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 339