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CD81 antibody

Anti-CD81 antibody used in Western Blot (WB). GTX31381
Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX31381
Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX31381
Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX31381
Anti-CD81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31381

Cat No. GTX31381

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2.5 μg/mL
ICC/IF Assay dependent
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

A549 Cell Slide(GTX25527) , RAW 264.7 Cell Lysate(GTX27187) , Human Lung Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81.The immunogen is located within amino acids 30 - 80 of CD81.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD81 molecule , CVID6 , S5.7 , TAPA1 , TSPAN28

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-CD81 antibody used in Western Blot (WB). GTX31381

GTX31381 WB Image

WB analysis of human lung tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31381 CD81 antibody.
Working concentration : 1 μg/ml

Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX31381

GTX31381 IHC-P Image

IHC-P analysis of human brain tissue using GTX31381 CD81 antibody.
Working concentration : 10 μg/ml

Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX31381

GTX31381 IHC-P Image

IHC-P analysis of human brain tissue using GTX31381 CD81 antibody.
Working concentration : 2 μg/ml

Anti-CD81 antibody used in IHC (Paraffin sections) (IHC-P). GTX31381

GTX31381 IHC-P Image

IHC-P analysis of human lung tissue using GTX31381 CD81 antibody.
Working concentration : 5 μg/ml

Anti-CD81 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31381

GTX31381 ICC/IF Image

ICC/IF analysis of A549 cells using GTX31381 CD81 antibody.
Working concentration : 20 μg/ml

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339