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CD86 antibody [BU63] (FITC)

Anti-CD86 antibody [BU63] (FITC) used in Flow cytometry (FACS). GTX74651

Cat No. GTX74651

Host

Mouse

Clonality

Monoclonal

Clone Name

BU63

Isotype

IgG1

Application

FACS

Reactivity

Human
Package
100 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS Neat

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide, 1% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human peripheral blood lymphocytes.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cd86 Molecule , B7-2 , B7.2 , B70 , Cd28Lg2 , Lab72 , Cd86

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CD86 antibody [BU63] (FITC) used in Flow cytometry (FACS). GTX74651

GTX74651 FACS Image

Figure A. RPE conjugated Mouse anti Human HLA DP/DQ/DR (GTX76200) and FITC conjugated Mouse IgG1 isotype control (GTX76638). Figure B. RPE conjugated Mouse anti Human HLA DP/DQ/DR (GTX76200) and FITC conjugated Mouse anti Human CD86 (GTX74651). All experiments performed on human Peripheral blood mononuclear cells.

REFERENCE

There are currently no references for CD86 antibody [BU63] (FITC) (GTX74651). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD86 antibody [BU63] (FITC) (GTX74651). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf