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CD86 antibody [BU63] (PE)

Cat. No. GTX74652

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BU63

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 test ($489)
See all CD86 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat-1/10

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 5% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human peripheral blood lymphocytes.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

RRID

AB_376397

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

REFERENCE

Clone Reference

Manferdini C et al. Biology (Basel) 2020; 9 (12) Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
Tsai PY et al. Int J Mol Sci 2019; 20 (23) NLRP7 Is Involved in the Differentiation of the Decidual Macrophages.
Carbone F et al. Int J Cardiol 2018; (Epub) Serum levels of osteopontin predict major adverse cardiovascular events in patients with severe carotid artery stenosis.
Wang LT et al. Cancer Res 2017; (Epub) Intestine-specific homeobox gene ISX integrates interleukin-6 signaling, tryptophan catabolism and immune suppression.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489