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CD86 antibody [BU63]

Anti-CD86 antibody [BU63] used in IHC (Paraffin sections) (IHC-P). GTX34571
Anti-CD86 antibody [BU63] used in Flow cytometry (FACS). GTX34571
Anti-CD86 antibody [BU63] used in Flow cytometry (FACS). GTX34571
Anti-CD86 antibody [BU63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34571

Cat No. GTX34571

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BU63

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($339)
See all CD86 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 2-4μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ARH-77 (B-lymphoblastoid cell line)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

B7.2 , B70 , B72 , CD28LG2 , CD86 , CD86 molecule , LAB72 , B7-2

Cellular Localization

Cell membrane

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CD86 antibody [BU63] used in IHC (Paraffin sections) (IHC-P). GTX34571

GTX34571 IHC-P Image

IHC-P analysis of human esophageal tumor tissue using GTX34571 CD86 antibody [BU63].

Anti-CD86 antibody [BU63] used in Flow cytometry (FACS). GTX34571

GTX34571 FACS Image

FACS analysis of human PBMCs using GTX34571 CD86 antibody [BU63].
Red : Primary antibody
Green : Isotype control

Anti-CD86 antibody [BU63] used in Flow cytometry (FACS). GTX34571

GTX34571 FACS Image

FACS analysis of PFA-fixed Ramos cells using GTX34571 CD86 antibody [BU63].
Blue : Primary antibody
Red : Isotype control

Anti-CD86 antibody [BU63] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34571

GTX34571 ICC/IF Image

ICC/IF analysis of PFA-fixed Ramos cells using GTX34571 CD86 antibody [BU63].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339