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CD86 antibody [BU63]

Cat. No. GTX74650

Reference 14

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BU63

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS, IP

Reactivity

 Human
Package
200 μg ($459)
See all CD86 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr 1/20-1/100
FACS 1/20-1/100
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human peripheral blood lymphocytes.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376395

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

REFERENCE

Application Reference

Manferdini C et al. Biology (Basel) 2020; 9 (12) Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
Tsai PY et al. Int J Mol Sci 2019; 20 (23) NLRP7 Is Involved in the Differentiation of the Decidual Macrophages.
Carbone F et al. Int J Cardiol 2018; (Epub) Serum levels of osteopontin predict major adverse cardiovascular events in patients with severe carotid artery stenosis.
Reactivity : Human
Wang LT et al. Cancer Res 2017; (Epub) Intestine-specific homeobox gene ISX integrates interleukin-6 signaling, tryptophan catabolism and immune suppression.
Application : WB
Reactivity : Human

Clone Reference

McCarthy NE et al. J Immunol 2013; 191 (5):2752-63 Proinflammatory Vδ2+ T cells populate the human intestinal mucosa and enhance IFN-γ production by colonic αβ T cells.
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REVIEW

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Application Reference
Manferdini C et al. Biology (Basel) 2020; 9 (12) Impact of Isolation Procedures on the Development of a Preclinical Synovial Fibroblasts/Macrophages in an In Vitro Model of Osteoarthritis.
Tsai PY et al. Int J Mol Sci 2019; 20 (23) NLRP7 Is Involved in the Differentiation of the Decidual Macrophages.
Carbone F et al. Int J Cardiol 2018; (Epub) Serum levels of osteopontin predict major adverse cardiovascular events in patients with severe carotid artery stenosis.
Reactivity : Human
Wang LT et al. Cancer Res 2017; (Epub) Intestine-specific homeobox gene ISX integrates interleukin-6 signaling, tryptophan catabolism and immune suppression.
Application : WB
Reactivity : Human
Clone Reference
McCarthy NE et al. J Immunol 2013; 191 (5):2752-63 Proinflammatory Vδ2+ T cells populate the human intestinal mucosa and enhance IFN-γ production by colonic αβ T cells.
Silk KM et al. J Biomed Biotechnol 2012; 2012:172420 Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
Application : FACS
Reactivity : Human
Salte T et al. Microbes Infect 2011; 13 (3):276-83 Increased intracellular growth of Mycobacterium avium in HIV-1 exposed monocyte-derived dendritic cells.
Hovden AO et al. BMC Immunol 2011; 12:2 Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
Application : FACS
Goodyear O et al. Blood 2010; 116 (11):1908-18 Induction of a CD8+ T-cell response to the MAGE cancer testis antigen by combined treatment with * and sodium valproate in patients with acute myeloid leukemia and myelodysplasia.
Adler HS et al. J Immunol 2010; 184 (11):6025-34 Neuronal nitric oxide synthase modulates maturation of human dendritic cells.
Application : FACS
Lozanoska-Ochser B et al. J Immunol 2008; 181 (9):6109-16 Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
Application : FACS
Angel CE et al. J Immunol 2006; 176 (10):1398971 Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
Application : FACS
Hofmann-Wellenhof R et al. J Invest Dermatol 2004; 123 (4):781-7 Sunburn cell formation, dendritic cell migration, and immunomodulatory factor production after solar-simulated irradiation of sunscreen-treated human skin explants in vitro.
Application : ICC/IF
Zhan H et al. Invest Ophthalmol Vis Sci 2003; 44 (9):732951 The immunomodulatory role of human conjunctival epithelial cells.
Application : FACS
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459