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CD86 antibody [BU63]

Cat No. GTX74650

Host Mouse
Clonality Monoclonal
Clone Name BU63
Isotype IgG1
Application WB, ICC/IF, IHC-Fr, FACS, IP, IHC
Reactivity Human
Package
200 μg ($379)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr 1/20-1/100
FACS 1/20-1/100
IP Assay dependent
IHC Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human peripheral blood lymphocytes.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd86 Molecule,B7-2,B7.2,B70,Cd28Lg2,Lab72,Cd86

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

REFERENCE
REVIEW
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Application Reference
Clone Reference