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CD86 antibody [BU63]

Cat. No. GTX74650

Host

Mouse

Clonality

Monoclonal

Clone Name

BU63

Isotype

IgG1

Application

WB, ICC/IF, IHC-Fr, FACS, IP, IHC

Reactivity

Human
Package
200 μg ($459)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr 1/20-1/100
FACS 1/20-1/100
IP Assay dependent
IHC Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human peripheral blood lymphocytes.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376395

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

REFERENCE

REVIEW

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Application Reference
Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459