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CD86 antibody [C86/1146]

Anti-CD86 antibody [C86/1146] used in Western Blot (WB). GTX34569
Anti-CD86 antibody [C86/1146] used in IHC (Paraffin sections) (IHC-P). GTX34569
Anti-CD86 antibody [C86/1146] used in Flow cytometry (FACS). GTX34569

Cat No. GTX34569

Host Mouse
Clonality Monoclonal
Clone Name C86/1146
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 2-4ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human full-length CD86 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd86 Molecule,B7-2,B7.2,B70,Cd28Lg2,Lab72,Cd86

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES
Anti-CD86 antibody [C86/1146] used in Western Blot (WB). GTX34569

GTX34569 WB Image

Western Blot Analysis of Daudi Cell Lysate using CD86 Monoclonal Antibody (C86/1146).

Anti-CD86 antibody [C86/1146] used in IHC (Paraffin sections) (IHC-P). GTX34569

GTX34569 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with CD86 Monoclonal Antibody (C86/1146).

Anti-CD86 antibody [C86/1146] used in Flow cytometry (FACS). GTX34569

GTX34569 FACS Image

Flow Cytometry of human PBMC's using CD86 Monoclonal Antibody (C86/1146).