CD86 antibody [C86/2160R]

Cat. No. GTX02615

Host	Rabbit
Clonality	Monoclonal
Clone Name	C86/2160R
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μg ($529)

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Datasheet

View product citations for antibody GTX02615 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	1-2 μg/ml
IHC-P	1-2 μg/ml
FACS	1-2 μg/million cells
ELISA	2-4 μg/ml (for coating)
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes. ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Observed MW

65-70 kDa. The observed M.W. is based on the following publication. PMID: 28326017

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length CD86 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Gene ID: 942 CD86
UniProt: P42081 CD86

Research Area

DATA IMAGES

GTX02615 WB Image

WB analysis of Ramos whole cell lysate using GTX02615 CD86 antibody [C86/2160R].

GTX02615 IHC-P Image

IHC-P analysis of human colon tissue section using GTX02615 CD86 antibody [C86/2160R].

GTX02615 FACS Image

FACS analysis of Ramos cells using GTX02615 CD86 antibody [C86/2160R].
Blue : Primary antibody
Red : Isotype control

GTX02615 ICC/IF Image

ICC/IF analysis of Raji cells using GTX02615 CD86 antibody [C86/2160R].
Green : Primary antibody
Red : nuclear counterstain

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty