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CD86 antibody [SPM600]

Anti-CD86 antibody [SPM600] used in IHC (Paraffin sections) (IHC-P). GTX34570
Anti-CD86 antibody [SPM600] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34570

Cat. No. GTX34570

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM600

Isotype

 IgG1

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($529)
See all CD86 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 2-4μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length CD86 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B7.2 , B70 , B72 , CD28LG2 , CD86 , CD86 molecule , LAB72 , B7-2

Cellular Localization

Cell membrane

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CD86 antibody [SPM600] used in IHC (Paraffin sections) (IHC-P). GTX34570

GTX34570 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34570 CD86 antibody [SPM600].

Anti-CD86 antibody [SPM600] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34570

GTX34570 ICC/IF Image

ICC/IF analysis of Ramos cells using GTX34570 CD86 antibody [SPM600].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529