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CD86 antibody

Anti-CD86 antibody used in Western Blot (WB). GTX32507
Anti-CD86 antibody used in Western Blot (WB). GTX32507
Anti-CD86 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32507
Anti-CD86 antibody used in IHC (Paraffin sections) (IHC-P). GTX32507

Cat. No. GTX32507

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD86 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887660

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CD86 antibody used in Western Blot (WB). GTX32507

GTX32507 WB Image

WB analysis of THP-1 cell lysate using GTX32507 CD86 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD86 antibody used in Western Blot (WB). GTX32507

GTX32507 WB Image

WB analysis of various sample lysates using GTX32507 CD86 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD86 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32507

GTX32507 ICC/IF Image

ICC/IF analysis of Raw264.7 cells using GTX32507 CD86 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD86 antibody used in IHC (Paraffin sections) (IHC-P). GTX32507

GTX32507 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX32507 CD86 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Lombardo KA et al. J Pathol 2022; 256 (2) : 223-234 BCG invokes superior STING-mediated innate immune response over radiotherapy in a carcinogen murine model of urothelial cancer.
Hongmei Zhao et al. Bioengineered 2022; 13 (4) : 9928-9944 Sp1 transcription factor represses transcription of phosphatase and tensin homolog to aggravate lung injury in mice with type 2 diabetes mellitus-pulmonary tuberculosis.
Application : IHC-P
Carbone F et al. Vascul Pharmacol 2016; 85 : 57-65 Vitamin D receptor is expressed within human carotid plaques and correlates with pro-inflammatory * macrophages.
Application : IHC-Fr
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399