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CD86 antibody

Anti-CD86 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32507
Anti-CD86 antibody used in Western Blot (WB). GTX32507
Anti-CD86 antibody used in IHC (Paraffin sections) (IHC-P). GTX32507

Cat No. GTX32507

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD86 molecule , B7-2 , B7.2 , B70 , CD28LG2 , LAB72

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-CD86 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32507

GTX32507 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX32507 CD86 antibody.
Blue : DAPI

Anti-CD86 antibody used in Western Blot (WB). GTX32507

GTX32507 WB Image

WB analysis of THP-1 cell lysates using GTX32507 CD86 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD86 antibody used in IHC (Paraffin sections) (IHC-P). GTX32507

GTX32507 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX32507 CD86 antibody.
Dilution : 1:100

REFERENCE

REVIEW

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