GeneTex
United States (US)

CD89 antibody

GTX104902_40723_20150821_IHC-P_19011506_577.jpg
GTX104902_40723_WB_19011506_797.jpg

Cat No. GTX104902

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Raji , K562

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD89. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD89 antibody, FCAR antibody, immunoglobulin alpha Fc receptor antibody, Fc alpha receptor antibody, IgA Fc receptor antibody, "Fc fragment of IgA, receptor for antibody"

Cellular Localization

Isoform A , 1: Cell membrane; Single-pass type I membrane protein , 2: Cell membrane; Single-pass type I membrane protein , 3: Cell membrane; Single-pass type I membrane protein , Isoform B: Secreted , Isoform B-delta-S2: Secreted

Background

This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX104902_40723_20150821_IHC-P_19011506_577.jpg

GTX104902 IHC-P Image

CD89 antibody detects CD89 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CD89 antibody (GTX104902) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104902_40723_WB_19011506_797.jpg

GTX104902 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
B: K562
5% SDS PAGE
GTX104902 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169