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CD9 antibody [MM2/57]

Anti-CD9 antibody [MM2/57] used in IHC (Frozen sections) (IHC-Fr). GTX76184

Cat. No. GTX76184

Reference 16

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MM2/57

Isotype

 IgG2b

Application

 WB, IHC-P, IHC-Fr, FACS, Blocking

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Cat, Dog, Pig, Ferret, Horse, Llama, Mink, Rhesus Monkey
Package
200 μg ($459)
See all CD9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IHC-Fr 1/500-1/1,000
FACS 1/100-1/200
Blocking Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Product Note

This antibody recognizes a conserved epitope on CD9 present on a wide range of mammalian species.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human platelet membranes.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_377219

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD9 molecule , BTCC-1 , DRAP-27 , MIC3 , MRP-1 , TSPAN-29 , TSPAN29

Cellular Localization

Cell membrane , Membrane

Background

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-CD9 antibody [MM2/57] used in IHC (Frozen sections) (IHC-Fr). GTX76184

GTX76184 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX76184 CD9 antibody [MM2/57].

REFERENCE

Application Reference

Park SM et al. PLoS One 2021; 16 (7) : e0254657 Extracellular vesicles derived from DFO-preconditioned canine AT-MSCs reprogram macrophages into M2 phase.
Application : WB
Reactivity : Dog

Clone Reference

Esmaeili A et al. Sci Rep 2022; 12 (1):19827 Co-aggregation of MSC/chondrocyte in a dynamic 3D culture elevates the therapeutic effect of secreted extracellular vesicles on osteoarthritis in a rat model.
Hosseinzadeh M et al. Biomed Res Int 2021; 2021:9011548 Higher Chondrogenic Potential of Extracellular Vesicles Derived from Mesenchymal Stem Cells Compared to Chondrocytes-EVs In Vitro.
Ogunnaike M et al. Int J Pharm 2021; 610:121263 Bovine mammary alveolar MAC-T cells afford a tool for studies of bovine milk exosomes in drug delivery.
Lingjun Tong et al. Theranostics 2021; 11 (17):8570-8586 Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.
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REVIEW

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Application Reference
Park SM et al. PLoS One 2021; 16 (7):e0254657 Extracellular vesicles derived from DFO-preconditioned canine AT-MSCs reprogram macrophages into M2 phase.
Application : WB
Reactivity : Dog
Xinhui Liu et al. Front Pharmacol 2020; 11:622658 Involvement of Circulating Exosomal MicroRNAs in Jian-Pi-Yi-Shen Formula Protection Against Adenine-Induced Chronic Kidney Disease.
Application : WB
Reactivity : Rat
Gaballa R et al. Cancers (Basel) 2020; 12 (8) Exosomes-Mediated Transfer of Itga2 Promotes Migration and Invasion of Prostate Cancer Cells by Inducing Epithelial-Mesenchymal Transition.
Application : WB
Reactivity : Human
An JH et al. Sci Rep 2020; 10 (1):2115 TNF-α and INF-γ Primed Canine Stem Cell-Derived Extracellular Vesicles Alleviate Experimental Murine Colitis.
Application : WB
Reactivity : Mouse
An JH et al. BMC Vet Res 2018; 14 (1):354 Prostaglandin E2 secreted from feline adipose tissue-derived mesenchymal stem cells alleviate DSS-induced colitis by increasing regulatory T cells in mice.
Application : FACS
Reactivity : Mouse
Khatri M et al. Stem Cell Res Ther 2018; 9 (1):17 Mesenchymal stem cell-derived extracellular vesicles attenuate influenza virus-induced acute lung injury in a pig model.
Application : FACS
Reactivity : Pig
Zevian S et al. J Biol Chem 2011; 286 (9):7496-506 Structure-function analysis of tetraspanin CD151 reveals distinct requirements for tumor cell behaviors mediated by α3β1 versus α6β4 integrin.
Application : WB
Reactivity : Human
Chang Y et al. J Cell Sci 2007; 120 (Pt 17):3053-63 Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
Application : Blocking
Reactivity : Bovine
Clone Reference
Esmaeili A et al. Sci Rep 2022; 12 (1):19827 Co-aggregation of MSC/chondrocyte in a dynamic 3D culture elevates the therapeutic effect of secreted extracellular vesicles on osteoarthritis in a rat model.
Hosseinzadeh M et al. Biomed Res Int 2021; 2021:9011548 Higher Chondrogenic Potential of Extracellular Vesicles Derived from Mesenchymal Stem Cells Compared to Chondrocytes-EVs In Vitro.
Ogunnaike M et al. Int J Pharm 2021; 610:121263 Bovine mammary alveolar MAC-T cells afford a tool for studies of bovine milk exosomes in drug delivery.
Lingjun Tong et al. Theranostics 2021; 11 (17):8570-8586 Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.
An JH et al. PLoS One 2020; 15 (2):e0220756 TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis.
Tong L et al. Mol Nutr Food Res 2020; 64 (8):e1901251 Oral Administration of Bovine Milk-Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice.
Ju-Hyun An et al. bioRxiv 2019; (Epub) TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis.
He P et al. FEBS Open Bio 2013; Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9
Application : IHC-P
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459