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CD9 antibody

Anti-CD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135296
Anti-CD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135296
Anti-CD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX135296
Anti-CD9 antibody used in Western Blot (WB). GTX135296

Cat. No. GTX135296

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Predict Reactivity

Rat, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909839

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD9 molecule , BTCC-1 , DRAP-27 , MIC3 , MRP-1 , TSPAN-29 , TSPAN29

Cellular Localization

Cell membrane,Membrane

Background

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-CD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135296

GTX135296 ICC/IF Image

CD9 antibody detects CD9 protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD9 stained by CD9 antibody (GTX135296) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135296

GTX135296 ICC/IF Image

CD9 antibody detects CD9 protein at cell membrane by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD9 stained by CD9 antibody (GTX135296) diluted at 1:500.

Anti-CD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX135296

GTX135296 IHC-P Image

CD9 antibody detects CD9 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CD9 stained by CD9 antibody (GTX135296) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD9 antibody used in Western Blot (WB). GTX135296

GTX135296 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD9 antibody (GTX135296) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3) : e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
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REVIEW

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Package List Price ($)
$ 399
$ 169