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CD90 antibody

Anti-CD90 antibody used in Western Blot (WB). GTX130072
Anti-CD90 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130072
Anti-CD90 antibody used in Western Blot (WB). GTX130072
Anti-CD90 antibody used in IHC (Paraffin sections) (IHC-P). GTX130072
Anti-CD90 antibody used in Western Blot (WB). GTX130072
Anti-CD90 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130072

Cat. No. GTX130072

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD90 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

C8D30

Predict Reactivity

Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD90. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886168

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Thy-1 cell surface antigen , CD90 , CDw90

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Database

Research Area

DATA IMAGES

Anti-CD90 antibody used in Western Blot (WB). GTX130072

GTX130072 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD90 antibody (GTX130072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD90 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130072

GTX130072 IHC-Fr Image

CD90 antibody detects CD90 protein (arrow) by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: CD90 protein stained by CD90 antibody (GTX130072) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD90 antibody used in Western Blot (WB). GTX130072

GTX130072 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD90 antibody (GTX130072) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD90 antibody used in IHC (Paraffin sections) (IHC-P). GTX130072

GTX130072 IHC-P Image

CD90 antibody detects CD90 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Green: CD90 stained by CD90 antibody (GTX130072) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD90 antibody used in Western Blot (WB). GTX130072

GTX130072 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CD90 antibody (GTX130072) diluted at 1:1000.

Anti-CD90 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130072

GTX130072 ICC/IF Image

CD90 antibody detects CD90 protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD90 stained by CD90 antibody (GTX130072) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Li Q et al. PLoS Pathog 2015; 11 (7) : e1004999 THY-1 Cell Surface Antigen (CD90) Has an Important Role in the Initial Stage of Human Cytomegalovirus Infection.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169