GeneTex
United States (US)

CD97 antibody [C3], C-term

GTX108192_WB_19011507_675.jpg
GTX108192_39735_IFA_19011507_272.jpg
GTX108192_39735_IHC_19011507_394.jpg

Cat No. GTX108192

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD97. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adhesion G Protein-Coupled Receptor E5,Cd97,Tm7Ln1,Adgre5

Cellular Localization

Cell membrane , CD97 antigen subunit alpha: Secreted , extracellular space

Background

This gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108192_WB_19011507_675.jpg

GTX108192 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: HCT116
7.5% SDS PAGE
GTX108192 diluted at 1:1000

GTX108192_39735_IFA_19011507_272.jpg

GTX108192 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CD97(GTX108192) antibody at 1:200 dilution.

GTX108192_39735_IHC_19011507_394.jpg

GTX108192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV21G xenograft, using CD97(GTX108192) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169