GeneTex
United States (US)

CD97 antibody [N3C3]

GTX106040_40408_WB_19011506_461.jpg
GTX106040_40408_IHC_19011506_524.jpg

Cat No. GTX106040

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

A549 , HeLa
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD97. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TM7LN1 antibody, CD97 antibody, seven-span transmembrane protein antibody, seven transmembrane helix receptor antibody, leukocyte antigen CD97 antibody, CD97 antigen antibody, "seven-transmembrane, heterodimeric receptor associated with inflammation antibody", CD97 molecule antibody

Cellular Localization

Cell membrane , CD97 antigen subunit alpha: Secreted , extracellular space

Background

This gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106040_40408_WB_19011506_461.jpg

GTX106040 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX106040 diluted at 1:1000

GTX106040_40408_IHC_19011506_524.jpg

GTX106040 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CD97(GTX106040) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169