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CD97 antibody

Anti-CD97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48818
Anti-CD97 antibody used in Western Blot (WB). GTX48818

Cat. No. GTX48818

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Chimpanzee
Package
100 μg ($399)
See all CD97 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000 - 1:5,000
ICC/IF 1:1,000 - 1:5,000
ELISA 1:10,000 - 1:50,000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adhesion G protein-coupled receptor E5 , AA409984 , Cd97 , TM7LN1

Cellular Localization

Cell membrane

Background

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.

Database

Research Area

DATA IMAGES

Anti-CD97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48818

GTX48818 ICC/IF Image

Immunofluorescence Microscopy using GeneTex Protein A purified anti-CD97 antibody (GTX48818) shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:2,500 dilution of the primary antibody was used.

Anti-CD97 antibody used in Western Blot (WB). GTX48818

GTX48818 WB Image

WB analysis of COS cells overexpressing CD97 using GTX48818 CD97 antibody.
Lane1 : mock
Lane2 : CD97 protein overexpressed

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399