GeneTex

  • Country / Location Selection

United States (US)

CD99 antibody

Anti-CD99 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113359
Anti-CD99 antibody used in Western Blot (WB). GTX113359
Anti-CD99 antibody used in IHC (Paraffin sections) (IHC-P). GTX113359

Cat. No. GTX113359

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD99 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human CD99. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949935

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD99 molecule (Xg blood group) , HBA71 , MIC2 , MIC2X , MIC2Y , MSK5X

Cellular Localization

Membrane

Background

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD99 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113359

GTX113359 ICC/IF Image

Immunofluorescence analysis of methanol-fixed H1299, using CD99(GTX113359) antibody at 1:500 dilution.

Anti-CD99 antibody used in Western Blot (WB). GTX113359

GTX113359 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
15% SDS PAGE
GTX113359 diluted at 1:1000

Anti-CD99 antibody used in IHC (Paraffin sections) (IHC-P). GTX113359

GTX113359 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CD99(GTX113359) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for CD99 antibody (GTX113359). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CD99 antibody (GTX113359). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169