CDC2 antibody [2G9]

Cat. No. GTX60412

Host	Mouse
Clonality	Monoclonal
Clone Name	2G9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μl ($409)

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Datasheet
Document

View product citations for antibody GTX60412 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
ELISA	1/10000

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CDK1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Gene ID: 983 CDK1
UniProt: P06493 CDK1

Research Area

DATA IMAGES

GTX60412 ICC/IF Image

ICC/IF analysis of Eca109 cells using GTX60412 CDC2 antibody [2G9].
Green : CDC2
Red: Actin filaments

GTX60412 ELISA Image

ELISA analysis of antigen using GTX60412 CDC2 antibody [2G9].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60412 IHC-P Image

IHC-P analysis of ovarian cancer tissue (left) and lung cancer tissue (right) using GTX60412 CDC2 antibody [2G9].

GTX60412 WB Image

WB analysis of Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIH/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate using GTX60412 CDC2 antibody [2G9].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty