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CDC2 antibody [CDK1/873]

Anti-CDC2 antibody [CDK1/873] used in Western Blot (WB). GTX34577
Anti-CDC2 antibody [CDK1/873] used in IHC (Paraffin sections) (IHC-P). GTX34577

Cat No. GTX34577

Host

Mouse

Clonality

Monoclonal

Clone Name

CDK1/873

Isotype

IgG2a

Application

WB, IHC-P

Reactivity

Human, Bovine, Monkey, Mink
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 2-4μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Specificity/Sensitivity

This product does not react with Mouse,Rat,Drosophila,Saccharomyces cerevisiae,Xenopus.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human full-length CDK1 protein.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDC2,CDC28A,CDK1,P34CDC2,cyclin dependent kinase 1,cdc2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 antibody [CDK1/873] used in Western Blot (WB). GTX34577

GTX34577 WB Image

WB analysis of HeLa cell lysate using GTX34577 CDC2 antibody [CDK1/873].

Anti-CDC2 antibody [CDK1/873] used in IHC (Paraffin sections) (IHC-P). GTX34577

GTX34577 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34577 CDC2 antibody [CDK1/873].

REFERENCE

There are currently no references for CDC2 antibody [CDK1/873] (GTX34577). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CDC2 antibody [CDK1/873] (GTX34577). Be the first to share your experience with this product.
Package List Price ($)
$ 339