*Optimal dilutions/concentrations should be determined by the researcher.
|2-4μg/ml for 30 minutes at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
This product does not react with Mouse,Rat,Drosophila,Saccharomyces cerevisiae,Xenopus.
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant human full-length CDK1 protein.
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CDC2,CDC28A,CDK1,P34CDC2,cyclin dependent kinase 1,cdc2
Nucleus,Cytoplasm,Mitochondrion,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
GTX34577 WB Image
WB analysis of HeLa cell lysate using GTX34577 CDC2 antibody [CDK1/873].
GTX34577 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34577 CDC2 antibody [CDK1/873].