Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CDC2 antibody [N1], N-term (GTX108120) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: PC-12
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: CDC2 protein stained by CDC2 antibody [N1], N-term (GTX108120) diluted at 1:750.
Blue: Hoechst 33342 staining.

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX108120 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC2 antibody [N1], N-term (GTX108120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the 2022 in FEBS Open Bio. PMID: 34856073