GeneTex
United States (US)

CDC2 antibody [N1], N-term

GTX108120_39778_20141231_IHC_19011507_357.jpg
GTX108120_39778_WB_19011507_583.jpg
GTX108120_39778_WB_R_19011507_254.jpg
GTX108120_39778_42403_IFA_19011507_410.jpg
GTX108120_39778_20170811_WB_19011507_420.jpg
GTX108120_39778_WB_M_19011507_933.jpg

Cat No. GTX108120

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , NIH-3T3 , JC , BCL-1 , PC-12

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377391)

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CDC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 1,Cdc2,Cdc28A,P34Cdc2,Cdk1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108120_39778_20141231_IHC_19011507_357.jpg

GTX108120 IHC-P Image

CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CDC2 antibody [N1], N-term (GTX108120) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108120_39778_WB_19011507_583.jpg

GTX108120 WB Image

Sample (30 µg of whole cell lysate)
A: A549
B: H1299
C: HCT116
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108120_39778_WB_R_19011507_254.jpg

GTX108120 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108120_39778_42403_IFA_19011507_410.jpg

GTX108120 ICC/IF Image

CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: CDC2 protein stained by CDC2 antibody [N1], N-term (GTX108120) diluted at 1:750.
Blue: Hoechst 33342 staining.

GTX108120_39778_20170811_WB_19011507_420.jpg

GTX108120 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC2 antibody [N1], N-term (GTX108120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108120_39778_WB_M_19011507_933.jpg

GTX108120 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX108120 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169