Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2 antibody [SM01-44] (GTX01143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FACS analysis of Jurkat cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

ICC/IF analysis of PFA-fixed HepG2 cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

ICC/IF analysis of PFA-fixed HepG2 cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

ICC/IF analysis of PFA-fixed HeLa cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

IHC-P analysis of human tonsil tissue section using GTX01143 CDC2 antibody [SM01-44].