APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:5000 |
| 1:50-1:200 |
| 1:50-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin dependent kinase 1 , CDC2 , CDC28A , CDK1 , P34CDC2 , cdc2
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
![Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143](/templates/default/js/lightbox/images/loading.gif "Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143")
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GTX01143 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2 antibody [SM01-44] (GTX01143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01143 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX01143 CDC2 antibody [SM01-44].
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GTX01143 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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GTX01143 ICC/IF Image
ICC/IF analysis of PFA-fixed HepG2 cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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GTX01143 ICC/IF Image
ICC/IF analysis of PFA-fixed HepG2 cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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GTX01143 FACS Image
FACS analysis of Jurkat cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50
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REFERENCE
There are currently no references for CDC2 antibody [SM01-44] (GTX01143). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDC2 antibody [SM01-44] (GTX01143). Be the first to share your experience with this product.
![Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143](/upload/website/prouct_img/small/GTX01143/GTX01143_HM0610_20200228_WB_w_23053121_878.webp "Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in IHC (Paraffin sections) (IHC-P). GTX01143](/upload/website/prouct_img/small/GTX01143/GTX01143_20200303_IHC-P_573_w_23053121_728.webp "Anti-CDC2 antibody [SM01-44] used in IHC (Paraffin sections) (IHC-P). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143](/upload/website/prouct_img/small/GTX01143/GTX01143_20200303_ICC-IF_570_w_23053121_123.webp "Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143](/upload/website/prouct_img/small/GTX01143/GTX01143_20200303_ICC-IF_571_w_23053121_600.webp "Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143](/upload/website/prouct_img/small/GTX01143/GTX01143_20200303_ICC-IF_572_w_23053121_450.webp "Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Flow cytometry (FACS). GTX01143](/upload/website/prouct_img/small/GTX01143/GTX01143_20200303_FACS_574_w_23053121_208.webp "Anti-CDC2 antibody [SM01-44] used in Flow cytometry (FACS). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143](/upload/website/prouct_img/normal/GTX01143/GTX01143_HM0610_20200228_WB_w_23053121_878.webp "Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in IHC (Paraffin sections) (IHC-P). GTX01143](/upload/website/prouct_img/normal/GTX01143/GTX01143_20200303_IHC-P_573_w_23053121_728.webp "Anti-CDC2 antibody [SM01-44] used in IHC (Paraffin sections) (IHC-P). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143](/upload/website/prouct_img/normal/GTX01143/GTX01143_20200303_ICC-IF_570_w_23053121_123.webp "Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143](/upload/website/prouct_img/normal/GTX01143/GTX01143_20200303_ICC-IF_571_w_23053121_600.webp "Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143](/upload/website/prouct_img/normal/GTX01143/GTX01143_20200303_ICC-IF_572_w_23053121_450.webp "Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143")
![Anti-CDC2 antibody [SM01-44] used in Flow cytometry (FACS). GTX01143](/upload/website/prouct_img/normal/GTX01143/GTX01143_20200303_FACS_574_w_23053121_208.webp "Anti-CDC2 antibody [SM01-44] used in Flow cytometry (FACS). GTX01143")