GeneTex
  • Country / Location Selection

United States (US)

CDC2 antibody [SM01-44]

Recombinant antibody
Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143
Anti-CDC2 antibody [SM01-44] used in Flow cytometry (FACS). GTX01143
Anti-CDC2 antibody [SM01-44] used in IHC (Paraffin sections) (IHC-P). GTX01143
Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143
Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143
Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143

Cat. No. GTX01143

Host

Rabbit

Clonality

Monoclonal

Clone Name

SM01-44

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , CDK1 , P34CDC2 , cdc2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 antibody [SM01-44] used in Western Blot (WB). GTX01143

GTX01143 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2 antibody [SM01-44] (GTX01143) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDC2 antibody [SM01-44] used in Flow cytometry (FACS). GTX01143

GTX01143 FACS Image

FACS analysis of Jurkat cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-CDC2 antibody [SM01-44] used in IHC (Paraffin sections) (IHC-P). GTX01143

GTX01143 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01143 CDC2 antibody [SM01-44].

Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143

GTX01143 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143

GTX01143 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-CDC2 antibody [SM01-44] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01143

GTX01143 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX01143 CDC2 antibody [SM01-44].
Red : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for CDC2 antibody [SM01-44] (GTX01143). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CDC2 antibody [SM01-44] (GTX01143). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399