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CDC2 antibody

Anti-CDC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50559
Anti-CDC2 antibody used in Western Blot (WB). GTX50559
Anti-CDC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50559

Cat. No. GTX50559

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 13~17 (G-T-Y-G-V) derived from human CDC2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50559

GTX50559 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50559 CDC2 antibody.

Anti-CDC2 antibody used in Western Blot (WB). GTX50559

GTX50559 WB Image

WB analysis of extracts from HeLa and MCF cells using GTX50559 CDC2 antibody.

Anti-CDC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50559

GTX50559 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50559 CDC2 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for CDC2 antibody (GTX50559). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CDC2 antibody (GTX50559). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399