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CDC2 (phospho Thr161) antibody

Anti-CDC2 (phospho Thr161) antibody used in Western Blot (WB). GTX38597
Anti-CDC2 (phospho Thr161) antibody used in IHC (Paraffin sections) (IHC-P). GTX38597

Cat. No. GTX38597

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μl ($399)
See all CDC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

CDC2 (phospho-Thr161) antibody detects endogenous levels of CDC2 only when phosphorylated at threonine 161.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of threonine161 (T -Y-T(p)-H-E).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 (phospho Thr161) antibody used in Western Blot (WB). GTX38597

GTX38597 WB Image

WB analysis of COLO205 cell (Lane 1 and 2) and K562 cell (Lane 3) lysates using GTX38597 CDC2 (phospho Thr161) antibody.

Anti-CDC2 (phospho Thr161) antibody used in IHC (Paraffin sections) (IHC-P). GTX38597

GTX38597 IHC-P Image

IHC-P analysis of human breast cacinoma tissue using GTX38597 CDC2 (phospho Thr161) antibody.

REFERENCE

Application Reference

YY Hsieh et al. Mol Oncol 2023; Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399