APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Product Note
CDC2 (phospho-Thr161) antibody detects endogenous levels of CDC2 only when phosphorylated at threonine 161.
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of threonine161 (T -Y-T(p)-H-E).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cyclin dependent kinase 1 , CDC2 , CDC28A , P34CDC2
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
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GTX38597 WB Image
WB analysis of COLO205 cell (Lane 1 and 2) and K562 cell (Lane 3) lysates using GTX38597 CDC2 (phospho Thr161) antibody.
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GTX38597 IHC-P Image
IHC-P analysis of human breast cacinoma tissue using GTX38597 CDC2 (phospho Thr161) antibody.
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REFERENCE
There are currently no references for CDC2 (phospho Thr161) antibody (GTX38597). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDC2 (phospho Thr161) antibody (GTX38597). Be the first to share your experience with this product.
