*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:1000
|1:50 - 1:100
Not tested in other applications.
Mouse, Rat(>80% identity)
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
0.02% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of threonine161 (T-Y-Tp-H-E).
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CDC2,CDC28A,CDK1,P34CDC2,cyclin dependent kinase 1,cdc2
Nucleus,Cytoplasm,Mitochondrion,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
GTX79115 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX79115 CDC2 (phospho Thr161) antibody.
GTX79115 WB Image
WB analysis of COLO205 (Lane 1 and 2) and K562 cell (Lane 3) lysates using GTX79115 CDC2 (phospho Thr161) antibody.
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