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CDC2 (phospho Thr161) antibody

Anti-CDC2 (phospho Thr161) antibody used in IHC (Paraffin sections) (IHC-P). GTX79115
Anti-CDC2 (phospho Thr161) antibody used in Western Blot (WB). GTX79115

Cat. No. GTX79115

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all CDC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of threonine161 (T-Y-Tp-H-E).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11176379

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDC2,CDC28A,CDK1,P34CDC2,cyclin dependent kinase 1,cdc2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Cytoskeleton,Microtubule organizing center,Centrosome,Spindle

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-CDC2 (phospho Thr161) antibody used in IHC (Paraffin sections) (IHC-P). GTX79115

GTX79115 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79115 CDC2 (phospho Thr161) antibody.

Anti-CDC2 (phospho Thr161) antibody used in Western Blot (WB). GTX79115

GTX79115 WB Image

WB analysis of COLO205 (Lane 1 and 2) and K562 cell (Lane 3) lysates using GTX79115 CDC2 (phospho Thr161) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399